The particle size, concentration, and Zeta potential of extracellular vesicles (EVs) are crucial parameters reflecting their biophysical characteristics and biological functions. The size of EVs depends on the originating cells, carriers, and biogenesis pathways, ranging in diameter from 30nm to ...
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Introduction of ChIP-Seq
Introduction of ChIP-Seq Chromatin immunoprecipitation sequencing (ChIP-Seq) stands as a cornerstone in contemporary genomics, revered for its prowess in mapping intricate protein-DNA interactions across the genome. Integrating the precision of chromatin immunoprecipitation (ChIP) with the robust throughput of next-generation sequencing ...
The Applications of DAP-seq Technology
Transcription factors (TFs) binding to specific locations in the genome influence dynamic changes in transcription levels. Disruption of TF binding sites (TFBS) correlates with phenotypic diversity and disease states, including agriculturally important adaptive traits and various diseases like cancer. Characterizing ...
Biological Function and Sequencing Technologies of m1A RNA Modification
Introduction to N1-methyladenosine (m1A) Sequencing In eukaryotic cells, N1-methyladenosine (m1A) is found in thousands of different gene transcripts (from yeast to mammals). The majority of m1A methylation modifications occur in the 5’UTR region of mRNA, and it is a common ...
RNA Sequencing in Drug Research and Development: Introduction, Advantages, and Applications
Overview In modern drug discovery and development, the research and development (R&D) process is a difficult task. The biological system is complex, which is one of the purposes. The human genome contains 20,000–25,000 genes. Traditional test methods for measuring gene ...
Summary of Protein Phosphorylation Detection Methods
Protein kinases operate by transferring a phosphate group from ATP onto serine, threonine, or tyrosine residues on a protein polypeptide substrate, thereby directly influencing its activity and function. Radioactive studies have demonstrated that approximately 30% of proteins in eukaryotic cells ...
Citrullination in Disease Proteomics
Proteomics, a dynamic field of study, is significantly influenced by post-translational modifications (PTMs). These modifications play a crucial role in finely tuning protein structure, function, stability, cellular localization, and enzymatic activity. Among these, citrullination, a specific PTM, involves the conversion ...
Overview of DNA Methylation Sequencing Methods
Background Introduction of DNA Methylation Research DNA methylation is a form of DNA chemical modification that can regulate genomic function without altering the primary structure of DNA, causing changes in chromatin structure, DNA stability, and DNA protein interaction patterns, thereby affecting ...
DAP-seq: Principles, Workflow and Analysis
Overview of DAP-seq The DNA Affinity Purification Sequencing (DAP-seq) technique is an innovative method aimed at exploring the interactions between transcription factors (TFs) and genomic DNA. By simulating the interaction between TFs and their DNA binding sites in vitro, this technology reveals ...
Applications of Lipidomics in Nutrition and Health
Lipids can influence lipid metabolic pathways in the body. Objective and accurate identification of potential lipid biomarkers in the process from health to disease progression, so as to develop dietary guidelines for disease prevention and health maintenance, is of great ...