Overview of PacBio sequencing Pacific Biosciences (PacBio) sequencing embodies a vital third-generation sequencing method, in the technical parlance, dubbed “Single Molecule Real-Time (SMRT) DNA Sequencing”. This direct approach circumvents the requirement for DNA amplification, thereby facilitating prompt sequence reading. Contrasting ...
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The Advantages and Workflow of 2b-RAD
What is RAD-seq? Nowadays, the application of reduced-representation sequencing technology in large-scale and high-throughput SNP genotyping is a hot area of research. Restriction enzymes are employed to fragment genomic DNA, then some characteristic fragments are selected and subjected to the ...
How Clinic Management Software Can Boost Your Healthcare Business
The healthcare sector is rapidly shifting towards digitization, and one of the standout tools driving this change is Clinic Management Software (CMS). Whether you’re running a solo medical practice or managing a network of clinics, this technology can transform the ...
WGBS Data Analysis: Workflow and Performance Evaluation Analysis Software
DNA methylation is related to transcription regulation, genome imprinting, stem cell differentiation, embryonic development and inflammation. Abnormal DNA methylation may reveal disease states, including cancer and nervous system diseases. Therefore, the distribution and locus of 5- methylcytosine (5mC) in human ...
Decoding RNA Acetylation through Workflow, Analysis, and Future Perspectives of acRIP-seq
Similar to the widely studied epigenome, the “epitranscriptome” generated by post-transcriptional ribonucleoside modifications embeds a complex layer of regulation in RNA structure and function. Even though it has been shown that messenger RNA (mRNA) modifications can affect post-transcriptional metabolism by ...
Decoding RNA Acetylation through Workflow, Analysis, and Future Perspectives of acRIP-seq
Similar to the widely studied epigenome, the “epitranscriptome” generated by post-transcriptional ribonucleoside modifications embeds a complex layer of regulation in RNA structure and function. Even though it has been shown that messenger RNA (mRNA) modifications can affect post-transcriptional metabolism by ...
RRBS for Detecting DNA Methylation
In the burgeoning field of epigenetics, DNA methylation has emerged as a pivotal mechanism in the regulation of gene expression, cell differentiation, development, and disease pathogenesis. Reduced Representation Bisulfite Sequencing (RRBS) has garnered significant attention in recent years as an ...
ATAC-seq – A Tool for Analyzing Epigenetics
Chromatin Dynamics and Gene Regulation in Eukaryotic Cells The total length of DNA within human cells approximates two meters and necessitates complex folding processes to be accommodated within the nucleus. During this dynamic folding process, chromatin exhibits heterogeneous structural states, ...
Advancing Detection and Research of Chimeric RNA and Sequencing Technologies
What is Chimeric RNA? Chimeric RNA, also known as fusion RNA, refers to RNA molecules that are formed as a result of the fusion of two or more different RNA transcripts. Chimeric RNA, a unique class of RNA molecules formed ...
Introduction, Applications, and Technologies of Single-Cell RNA Sequencing
Introduction to Single-Cell RNA Sequencing RNA sequencing (RNA-seq) is a genomic technique for detecting and quantifying messenger RNA molecules in biological samples, which is useful for studying cellular responses. In recent years, RNA-seq has fueled a lot of medical discoveries ...