Introduction of ChIP-Seq Chromatin immunoprecipitation sequencing (ChIP-Seq) stands as a cornerstone in contemporary genomics, revered for its prowess in mapping intricate protein-DNA interactions across the genome. Integrating the precision of chromatin immunoprecipitation (ChIP) with the robust throughput of next-generation sequencing ...
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The Applications of DAP-seq Technology
Transcription factors (TFs) binding to specific locations in the genome influence dynamic changes in transcription levels. Disruption of TF binding sites (TFBS) correlates with phenotypic diversity and disease states, including agriculturally important adaptive traits and various diseases like cancer. Characterizing ...
Biological Function and Sequencing Technologies of m1A RNA Modification
Introduction to N1-methyladenosine (m1A) Sequencing In eukaryotic cells, N1-methyladenosine (m1A) is found in thousands of different gene transcripts (from yeast to mammals). The majority of m1A methylation modifications occur in the 5’UTR region of mRNA, and it is a common ...
RNA Sequencing in Drug Research and Development: Introduction, Advantages, and Applications
Overview In modern drug discovery and development, the research and development (R&D) process is a difficult task. The biological system is complex, which is one of the purposes. The human genome contains 20,000–25,000 genes. Traditional test methods for measuring gene ...
Overview of DNA Methylation Sequencing Methods
Background Introduction of DNA Methylation Research DNA methylation is a form of DNA chemical modification that can regulate genomic function without altering the primary structure of DNA, causing changes in chromatin structure, DNA stability, and DNA protein interaction patterns, thereby affecting ...
DAP-seq: Principles, Workflow and Analysis
Overview of DAP-seq The DNA Affinity Purification Sequencing (DAP-seq) technique is an innovative method aimed at exploring the interactions between transcription factors (TFs) and genomic DNA. By simulating the interaction between TFs and their DNA binding sites in vitro, this technology reveals ...
Whole Exome Sequencing Trends in 2024
Whole exome sequencing overview Exons represent the protein-coding regions within the genome, and whole-exome sequencing (WES) entails sequencing all these regions. Research indicates that over 85% of base mutations occur within exonic regions, underscoring the crucial role of WES in unraveling genetic ...
Comprehensive Review of Circle Sequencing
What is Circle Sequencing? Circle sequencing represents a pivotal progression in library preparation protocols for high-throughput sequencing, effectively expediting advancements within the expansive domain of genomic research. This innovative protocol diverges from the conventional linear sequencing methodologies, placing emphasis instead ...